Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D

F. Takusagawa, B. M. Goldstein, S. Youngster, R. A. Jones, H. M. Berman

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Abstract

Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P212121 and a = 95.6, b = 42.7, and c = 40.8 Å. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)4714-4715
Number of pages2
JournalJournal of Biological Chemistry
Volume259
Issue number8
StatePublished - 1984

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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