Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D

F. Takusagawa; B. M. Goldstein; S. Youngster; R. A. Jones; H. M. Berman

Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D

F. Takusagawa, B. M. Goldstein, S. Youngster, R. A. Jones, H. M. Berman

School of Arts and Sciences, Chemistry & Chemical Biology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P2₁2₁2₁ and a = 95.6, b = 42.7, and c = 40.8 Å. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.

Original language	English (US)
Pages (from-to)	4714-4715
Number of pages	2
Journal	Journal of Biological Chemistry
Volume	259
Issue number	8
State	Published - 1984

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

Cite this

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title = "Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D",

abstract = "Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P212121 and a = 95.6, b = 42.7, and c = 40.8 {\AA}. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.",

author = "F. Takusagawa and Goldstein, {B. M.} and S. Youngster and Jones, {R. A.} and Berman, {H. M.}",

year = "1984",

language = "English (US)",

volume = "259",

pages = "4714--4715",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

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T1 - Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D

AU - Takusagawa, F.

AU - Goldstein, B. M.

AU - Youngster, S.

AU - Jones, R. A.

AU - Berman, H. M.

PY - 1984

Y1 - 1984

N2 - Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P212121 and a = 95.6, b = 42.7, and c = 40.8 Å. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.

AB - Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P212121 and a = 95.6, b = 42.7, and c = 40.8 Å. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.

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C2 - 6715318

AN - SCOPUS:0021337228

SN - 0021-9258

VL - 259

SP - 4714

EP - 4715

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -

Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D

Abstract

All Science Journal Classification (ASJC) codes

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