Crystallographic Analysis of C-C-A-A-G-C-T-T-G-G and Its Implications for Bending in B-DNA

Kazimierz Grzeskowiak, David S. Goodsell, Maria Kaczor-Grzeskowiak, Duilio Cascio, Richard E. Dickerson

Research output: Contribution to journalArticle

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Abstract

Stacked B-DNA double helices of sequence C-C-A-A-G-C-T-T-G-G exhibit the same 23° bend at -T-G-G C-C-A- across the nonbonded junction between helices that is observed in the middle of the decamer helix of sequence C-A-T-G-G-C-C-A-T-G, even though the space group (hexagonal vs orthorhombic), crystal packing, and connectedness at the center of the bent segment are quite different. An identical bend occurs across the interhelix junction of every monoclinic crystal structure of sequence C-C-A-x-x-x-x-T-G-G, suggesting that T-G-G-C-C-A constitutes a natural bending element in B-DNA. The bend occurs by rolling stacked base pairs about their long axes; there is no “tilt” component. Of the three possible models for A-tract bending—bent-A-tract, junction bends, or bent-non-A—which cannot be distinguished by solution measurements, all crystallographic evidence over the past 10 years unanimously supports the non-A regions as the actual bending loci.

Original languageEnglish (US)
Pages (from-to)8923-8931
Number of pages9
JournalBiochemistry
Volume32
Issue number34
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Grzeskowiak, K., Goodsell, D. S., Kaczor-Grzeskowiak, M., Cascio, D., & Dickerson, R. E. (1993). Crystallographic Analysis of C-C-A-A-G-C-T-T-G-G and Its Implications for Bending in B-DNA. Biochemistry, 32(34), 8923-8931. https://doi.org/10.1021/bi00085a025