CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli

Kunitoshi Yamanaka, Weidong Zheng, Elliott Crooke, Yuh Hwa Wang, Masayori Inouye

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

CspD is a stationary phase-induced, stress response protein in the CspA family of Escherichia coli. Here, we demonstrate that overproduction of CspD is lethal, with the cells displaying a morphology typical of cells with impaired DNA replication. CspD consists mainly of β-strands, and the purified protein exists exclusively as a dimer and binds to single-stranded (ss)DNA and RNA in a dose-dependent manner without apparent sequence specificity. CsdD effectively inhibits both the initiation and the elongation steps of minichromosome replication in vitro. Electron microscopic studies revealed that CspD tightly packs ssDNA, resulting in structures distinctly different from those of SSB-coated DNA. We propose that CspD dimers, with two independent β-sheets interacting with ssDNA, function as a novel inhibitor of DNA replication and play a regulatory role in chromosomal replication in nutrient-depleted cells.

Original languageEnglish (US)
Pages (from-to)1572-1584
Number of pages13
JournalMolecular microbiology
Volume39
Issue number6
DOIs
StatePublished - Apr 17 2001

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DNA Replication
Escherichia coli
Single-Stranded DNA
Heat-Shock Proteins
RNA
Electrons
Food
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Yamanaka, Kunitoshi ; Zheng, Weidong ; Crooke, Elliott ; Wang, Yuh Hwa ; Inouye, Masayori. / CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli. In: Molecular microbiology. 2001 ; Vol. 39, No. 6. pp. 1572-1584.
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CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli. / Yamanaka, Kunitoshi; Zheng, Weidong; Crooke, Elliott; Wang, Yuh Hwa; Inouye, Masayori.

In: Molecular microbiology, Vol. 39, No. 6, 17.04.2001, p. 1572-1584.

Research output: Contribution to journalArticle

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