Cultivation of immature astrocytes of mouse cerebellum in a serum-free, hormonally defined medium. Appearance of the mature astrocytic phenotype after addition of serum

Günther Fischer, Achim Leutz, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

A hormonally defined culture medium is described which supports survival and proliferation of astroglia from primary cultures of early postnatal mouse cerebellum. This medium consists of bovine serum albumin, insulin, transferrin, selenium, hyaluronic acid, protease inhibitor aprotinin, and epidermal growth factor. Trypsin-dissociated single cerebellar cell suspensions are plated in this medium on poly-l-lysine-coated glass coverslips and maintained for two weeks before subcultivation. After subcultivation into defined medium more than 99% of all cells are vimentin-positive and fibronectin- and almost completely glial fibrillary acid (GFA) protein-negative, indicating that these cells are less mature astrocytes. After replacement of defined medium by culture medium containing 10% horse serum, expression of GFA protein is detectable in addition to vimentin by indirect immunofluorescence.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalNeuroscience Letters
Volume29
Issue number3
DOIs
StatePublished - Apr 26 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • astrocytes
  • cell culture
  • cell type-specific neural antigens
  • differentiation
  • epidermal growth factor
  • mouse cerebellum
  • serum-free medium

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