TY - JOUR
T1 - Culture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes
T2 - A comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridge
AU - Voordeckers, James W.
AU - Do, My H.
AU - Hügler, Michael
AU - Ko, Vivian
AU - Sievert, Stefan M.
AU - Vetriani, Costantino
N1 - Funding Information:
Acknowledgments We wish to thank the crew of R/V Atlantis, and the crew and pilots of the deep-submergence vehicle Alvin, for their skilled operations at sea. We thank Nicole LeFur for technical assistance and Ken Takai (Japan Agency for Marine-Earth Science and Technology) for kindly making genomic DNA of H. thermo-phila, L. acidiphila, S. autotrophica, and S. lithotrophicum available to us. This research was supported by NSF grants MCB 04–56676 (C.V.), OCE 03–27353 (C.V.), MCB 04–56689 (S.M.S.), a grant from the New Jersey Agricultural Experiment Station to C.V., and a NIH Ph.D. Training Program in Biotechnology Fellowship (NIH NIGMS 5 T32 GM08339) to J.V. M.H. was supported through a postdoctoral scholarship from the Woods Hole Oceanographic Institution.
PY - 2008/9
Y1 - 2008/9
N2 - The bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were investigated using an integrated culture-dependent and independent approach. Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria and reference strains originally isolated from several different deep-sea vents. Our data showed that the three MAR hydrothermal vent chimneys investigated in this study host very different microbial assemblages. The microbial community of the Rainbow chimney was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate-reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that thermophilic sulfate reduction might also be occurring at this site. The Logatchev bacterial community included several sequences related to mesophilic sulfur-oxidizing bacteria, while the archaeal component of this chimney was dominated by sequences related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from natural microbial communities suggested that Epsilonproteobacteria were the dominant primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur chimney.
AB - The bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were investigated using an integrated culture-dependent and independent approach. Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria and reference strains originally isolated from several different deep-sea vents. Our data showed that the three MAR hydrothermal vent chimneys investigated in this study host very different microbial assemblages. The microbial community of the Rainbow chimney was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate-reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that thermophilic sulfate reduction might also be occurring at this site. The Logatchev bacterial community included several sequences related to mesophilic sulfur-oxidizing bacteria, while the archaeal component of this chimney was dominated by sequences related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from natural microbial communities suggested that Epsilonproteobacteria were the dominant primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur chimney.
KW - ANME
KW - ATP citrate lyase
KW - Aquificales
KW - Black smoker
KW - Deep-sea vent
KW - Epsilonproteobacteria
KW - rTCA cycle
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U2 - 10.1007/s00792-008-0167-5
DO - 10.1007/s00792-008-0167-5
M3 - Article
C2 - 18523725
AN - SCOPUS:51049089133
SN - 1431-0651
VL - 12
SP - 627
EP - 640
JO - Extremophiles
JF - Extremophiles
IS - 5
ER -