Cytochemical probes for ultrastructural detection of Yin and Yang components of cell surface

F. C. Bratila, V. Muresan

Research output: Contribution to journalArticle

Abstract

Two cytochemical techniques were used for the characterization and ultrastructural detection of Yin and Yang components of the endothelial surface of rat pancreatic capillaries, chosen as model system for an investigation at subcellular level. Horseradish peroxidase hydrazide was employed to demonstrate sialyl residues of sodium periodate-oxidized glycoconjugates. Being a carbohydrate of acidic character, sialic acid was considered to be one of the Yang-type components of cell surfaces. Free amino groups of basic proteins of a Yin character were detected with oxidized horseradish peroxidase. The distribution of Yin and Yang components on the endothelial surface, as detected with the above procedures, reflected the morphological and functional specialization of the cell. Although nonhomogeneously distributed, the two groups of molecules coexisted in most domains of the endothelial surface, generating a characteristic pattern of Yin and Yang. It is concluded that the alterations of the normal distribution pattern of Yin and Yang components at cellular level may be a consequence of an energetic imbalance of the whole organism.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalAmerican Journal of Acupuncture
Volume18
Issue number1
StatePublished - Jan 1 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine
  • Clinical Neurology

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