Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an interspecific Hordeum hybrid

T. J. Orton, R. P. Steidl

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Hybrids of Hordeum vulgare (HV) x H. jubatum (HJ) were synthesized for purposes of introgressive breeding, but were sterile and the recovery of pure diploid tillers by colchicine applications in vivo was difficult. Plant regeneration from colchicine-treated callus cultures of the hybrid (HV x HJ) was investigated as a means to produce high numbers of pure diploid, fertile intermediates. 10 of 50 plants regenerated in this manner exhibited variable chromosome numbers with means of approximately 37 (expected diploid number = 42). Cytological examinations of microsporogenesis in all such plants revealed a high incidence of bivalent formation at metaphase I (as compared to nearly complete asynapsis in the F1), but spindle and chromosome abnormalities in later meiotic stages led to complete sterility. Approximately 40% of HJ plants regenerated from colchicine-treated calli appeared to be pure tetraploids of high fertility. These techniques are hence useful for high frequency production of diploid or polyploid plants.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalTheoretical and Applied Genetics
Volume57
Issue number2
DOIs
StatePublished - Mar 1980
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Keywords

  • Barley
  • Callus culture
  • Cytogenetics
  • Hordeum hybrids
  • Introgressive breeding
  • Plant regeneration
  • Regenerates

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