Abstract
Hybrids of Hordeum vulgare (HV) x H. jubatum (HJ) were synthesized for purposes of introgressive breeding, but were sterile and the recovery of pure diploid tillers by colchicine applications in vivo was difficult. Plant regeneration from colchicine-treated callus cultures of the hybrid (HV x HJ) was investigated as a means to produce high numbers of pure diploid, fertile intermediates. 10 of 50 plants regenerated in this manner exhibited variable chromosome numbers with means of approximately 37 (expected diploid number = 42). Cytological examinations of microsporogenesis in all such plants revealed a high incidence of bivalent formation at metaphase I (as compared to nearly complete asynapsis in the F1), but spindle and chromosome abnormalities in later meiotic stages led to complete sterility. Approximately 40% of HJ plants regenerated from colchicine-treated calli appeared to be pure tetraploids of high fertility. These techniques are hence useful for high frequency production of diploid or polyploid plants.
Original language | English (US) |
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Pages (from-to) | 89-95 |
Number of pages | 7 |
Journal | Theoretical and Applied Genetics |
Volume | 57 |
Issue number | 2 |
DOIs | |
State | Published - Mar 1980 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
- Genetics
Keywords
- Barley
- Callus culture
- Cytogenetics
- Hordeum hybrids
- Introgressive breeding
- Plant regeneration
- Regenerates