Cytokines induce an early steroid resistance in airway smooth muscle cells: Novel role of interferon regulatory factor-1

Omar Tliba, Gautam Damera, Audreesh Banerjee, Su Gu, Hasna Baidouri, Stefan Keslacy, Yassine Amrani

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

We have previously shown that long-term treatment of airway smooth muscle (ASM) cells with a combination of TNF-α and IFN-γ impaired steroid anti-inflammatory action through the up-regulation of glucocorticoid receptor beta isoform (GRβ) (Mol Pharmacol 2006;69:588-596). We here found that steroid actions could also be suppressed by short-term exposure of ASM cells to TNF-α and IFN-γ (6 h) as shown by the abrogated glucocorticoid responsive element (GRE)-dependent gene transcription; surprisingly, neither GRα nuclear translocation nor GRβ expression was affected by cytokine mixture. The earlier induction of CD38, a molecule recently involved in asthma, seen with TNF-α and IFN-γ combination but not with cytokine alone, was also completely insensitive to steroid pretreatment. Chromatin- immunoprecipitation (IP) and siRNA strategies revealed not only increased binding of interferon regulatory factor 1 (IRF-1) transcription factor to CD38 promoter, but also its implication in regulating CD38 gene transcription. Interestingly, the capacity of fluticasone to completely inhibit TNF-α-induced IRF-1 expression, IRF-1 DNA binding, and transactivation activities was completely lost in cells exposed to TNF-α and IFN-γ in combination. This early steroid dysfunction seen with cytokine combination could be reproduced by enhancing IRF-1 cellular levels using constitutively active IRF-1, which dose-dependently inhibited GRE-dependent gene transcription. Consistently, reducing IRF-1 cellular levels using siRNA approach significantly restored steroid transactivation activities. Collectively, our findings demonstrate for the first time that IRF-1 is a novel alternative GRβ-independent mechanism mediating steroid dysfunction induced by pro-asthmatic cytokines, in part via the suppression of GRα activities.

Original languageEnglish (US)
Pages (from-to)463-472
Number of pages10
JournalAmerican journal of respiratory cell and molecular biology
Volume38
Issue number4
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Keywords

  • Asthma
  • Glucocorticoid
  • Inflammation
  • Mesenchymal cells
  • Transcription factor

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