Cytological analysis of meiosis in fixed Drosophila ovaries.

Kim S. McKim; Eric F. Joyce; Janet K. Jang

doi:10.1007/978-1-60761-103-5_12

Cytological analysis of meiosis in fixed Drosophila ovaries.

Kim S. McKim, Eric F. Joyce, Janet K. Jang

Research output: Contribution to journal › Review article › peer-review

20 Scopus citations

Abstract

Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.

Original language	English (US)
Pages (from-to)	197-216
Number of pages	20
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	558
DOIs	https://doi.org/10.1007/978-1-60761-103-5_12
State	Published - 2009

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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title = "Cytological analysis of meiosis in fixed Drosophila ovaries.",

abstract = "Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.",

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AB - Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.

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