De novo assembly of plasmids using yeast recombinational cloning

Ameya A. Mashruwala, Jeffrey M. Boyd

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-41
Number of pages9
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1373
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Genetics
  • Homologous recombination
  • Molecular biology
  • Molecular cloning
  • Staphylococcus
  • Yeast

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