@inbook{f4a09c08da70452d86c0abe31f54ca7b,
title = "De novo assembly of plasmids using yeast recombinational cloning",
abstract = "Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.",
keywords = "Genetics, Homologous recombination, Molecular biology, Molecular cloning, Staphylococcus, Yeast",
author = "Mashruwala, {Ameya A.} and Boyd, {Jeffrey M.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",
year = "2016",
doi = "10.1007/7651_2015_275",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "33--41",
booktitle = "Methods in Molecular Biology",
}