Dechlorinating bacteria are abundant but anaerobic dechlorination of weathered polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sediments is limited

Sanna Mäntynen, Anna Lea Rantalainen, Max Haggblom

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Abstract

The potential for microbial dechlorination of the weathered polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) was determined in sediments with historical contamination by the chlorophenol wood preservative Ky-5 and its associated dimeric impurities. Sediments were collected from four sites of the Kymijoki River in South-Eastern Finland located at 0, 20, 30, and 60 km downstream from the source of contamination, and at a reference site. We examined the congener profiles of historical PCDD/Fs, including non-2,3,7,8-substituted congeners, and determined the dechlorination potential in sediments at the different sites of the river. The measured mean total concentrations for 2,3,7,8-PCDD/Fs were extremely high, 1200 mg/kg dw, at the most contaminated site, Kuusankoski. The mean concentrations for the predominant 2,3,7,8-congeners were 1,2,3,4,6,7,8-HpCDF 780 mg/kg dw, and for OCDF 380 mg/kg dw at Kuusankoski. At all other study sites of the river the mean total concentrations for 2,3,7,8-PCDD/Fs varied between 9 and 96 mg/kg dw, (6–80 mg/kg dw for 1,2,3,4,6,7,8-HpCDF, 3–13 mg/kg dw for OCDF). The sediment PCDD/F composition was similar to that of Ky-5, indicating that no or only minimal biodegradation of PCDD/F congeners has occurred in the river sediments over the last few decades since the contamination events. Microbes capable of PCDD/F dechlorination were present at all study sites based on Dehalococcoides-like Chloroflexi community determination and dechlorination of spiked 1,2,3,4-tetrachlorodibenzofuran. However, no substantial changes in the relative abundances of PCDD/Fs were observed over 2.5 years in laboratory microcosm studies, indicating that anaerobic dechlorination of weathered PCDD/Fs was limited over the course of the experiment. Therefore, concentrations of weathered PCDD/Fs in the sediments of the Kymijoki River are expected to remain at the same level for decades or centuries with further migration towards the Baltic Sea.

Original languageEnglish (US)
Pages (from-to)560-568
Number of pages9
JournalEnvironmental Pollution
Volume231
DOIs
StatePublished - Dec 1 2017

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Dechlorination
Anaerobic Bacteria
Bacteria
Sediments
Rivers
Contamination
Chlorophenols
Biodegradation
Chloroflexi
dibenzo(1,4)dioxin
Polychlorinated Dibenzodioxins
Dibenzofurans
Wood
Impurities
Finland
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

@article{b86ded875adb4072aaa97ccee13937a2,
title = "Dechlorinating bacteria are abundant but anaerobic dechlorination of weathered polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sediments is limited",
abstract = "The potential for microbial dechlorination of the weathered polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) was determined in sediments with historical contamination by the chlorophenol wood preservative Ky-5 and its associated dimeric impurities. Sediments were collected from four sites of the Kymijoki River in South-Eastern Finland located at 0, 20, 30, and 60 km downstream from the source of contamination, and at a reference site. We examined the congener profiles of historical PCDD/Fs, including non-2,3,7,8-substituted congeners, and determined the dechlorination potential in sediments at the different sites of the river. The measured mean total concentrations for 2,3,7,8-PCDD/Fs were extremely high, 1200 mg/kg dw, at the most contaminated site, Kuusankoski. The mean concentrations for the predominant 2,3,7,8-congeners were 1,2,3,4,6,7,8-HpCDF 780 mg/kg dw, and for OCDF 380 mg/kg dw at Kuusankoski. At all other study sites of the river the mean total concentrations for 2,3,7,8-PCDD/Fs varied between 9 and 96 mg/kg dw, (6–80 mg/kg dw for 1,2,3,4,6,7,8-HpCDF, 3–13 mg/kg dw for OCDF). The sediment PCDD/F composition was similar to that of Ky-5, indicating that no or only minimal biodegradation of PCDD/F congeners has occurred in the river sediments over the last few decades since the contamination events. Microbes capable of PCDD/F dechlorination were present at all study sites based on Dehalococcoides-like Chloroflexi community determination and dechlorination of spiked 1,2,3,4-tetrachlorodibenzofuran. However, no substantial changes in the relative abundances of PCDD/Fs were observed over 2.5 years in laboratory microcosm studies, indicating that anaerobic dechlorination of weathered PCDD/Fs was limited over the course of the experiment. Therefore, concentrations of weathered PCDD/Fs in the sediments of the Kymijoki River are expected to remain at the same level for decades or centuries with further migration towards the Baltic Sea.",
author = "Sanna M{\"a}ntynen and Rantalainen, {Anna Lea} and Max Haggblom",
year = "2017",
month = "12",
day = "1",
doi = "10.1016/j.envpol.2017.08.050",
language = "English (US)",
volume = "231",
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journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Limited",

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TY - JOUR

T1 - Dechlorinating bacteria are abundant but anaerobic dechlorination of weathered polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sediments is limited

AU - Mäntynen, Sanna

AU - Rantalainen, Anna Lea

AU - Haggblom, Max

PY - 2017/12/1

Y1 - 2017/12/1

N2 - The potential for microbial dechlorination of the weathered polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) was determined in sediments with historical contamination by the chlorophenol wood preservative Ky-5 and its associated dimeric impurities. Sediments were collected from four sites of the Kymijoki River in South-Eastern Finland located at 0, 20, 30, and 60 km downstream from the source of contamination, and at a reference site. We examined the congener profiles of historical PCDD/Fs, including non-2,3,7,8-substituted congeners, and determined the dechlorination potential in sediments at the different sites of the river. The measured mean total concentrations for 2,3,7,8-PCDD/Fs were extremely high, 1200 mg/kg dw, at the most contaminated site, Kuusankoski. The mean concentrations for the predominant 2,3,7,8-congeners were 1,2,3,4,6,7,8-HpCDF 780 mg/kg dw, and for OCDF 380 mg/kg dw at Kuusankoski. At all other study sites of the river the mean total concentrations for 2,3,7,8-PCDD/Fs varied between 9 and 96 mg/kg dw, (6–80 mg/kg dw for 1,2,3,4,6,7,8-HpCDF, 3–13 mg/kg dw for OCDF). The sediment PCDD/F composition was similar to that of Ky-5, indicating that no or only minimal biodegradation of PCDD/F congeners has occurred in the river sediments over the last few decades since the contamination events. Microbes capable of PCDD/F dechlorination were present at all study sites based on Dehalococcoides-like Chloroflexi community determination and dechlorination of spiked 1,2,3,4-tetrachlorodibenzofuran. However, no substantial changes in the relative abundances of PCDD/Fs were observed over 2.5 years in laboratory microcosm studies, indicating that anaerobic dechlorination of weathered PCDD/Fs was limited over the course of the experiment. Therefore, concentrations of weathered PCDD/Fs in the sediments of the Kymijoki River are expected to remain at the same level for decades or centuries with further migration towards the Baltic Sea.

AB - The potential for microbial dechlorination of the weathered polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) was determined in sediments with historical contamination by the chlorophenol wood preservative Ky-5 and its associated dimeric impurities. Sediments were collected from four sites of the Kymijoki River in South-Eastern Finland located at 0, 20, 30, and 60 km downstream from the source of contamination, and at a reference site. We examined the congener profiles of historical PCDD/Fs, including non-2,3,7,8-substituted congeners, and determined the dechlorination potential in sediments at the different sites of the river. The measured mean total concentrations for 2,3,7,8-PCDD/Fs were extremely high, 1200 mg/kg dw, at the most contaminated site, Kuusankoski. The mean concentrations for the predominant 2,3,7,8-congeners were 1,2,3,4,6,7,8-HpCDF 780 mg/kg dw, and for OCDF 380 mg/kg dw at Kuusankoski. At all other study sites of the river the mean total concentrations for 2,3,7,8-PCDD/Fs varied between 9 and 96 mg/kg dw, (6–80 mg/kg dw for 1,2,3,4,6,7,8-HpCDF, 3–13 mg/kg dw for OCDF). The sediment PCDD/F composition was similar to that of Ky-5, indicating that no or only minimal biodegradation of PCDD/F congeners has occurred in the river sediments over the last few decades since the contamination events. Microbes capable of PCDD/F dechlorination were present at all study sites based on Dehalococcoides-like Chloroflexi community determination and dechlorination of spiked 1,2,3,4-tetrachlorodibenzofuran. However, no substantial changes in the relative abundances of PCDD/Fs were observed over 2.5 years in laboratory microcosm studies, indicating that anaerobic dechlorination of weathered PCDD/Fs was limited over the course of the experiment. Therefore, concentrations of weathered PCDD/Fs in the sediments of the Kymijoki River are expected to remain at the same level for decades or centuries with further migration towards the Baltic Sea.

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