Decorin activates the epidermal growth factor receptor and elevates cytosolic Ca2+ in A431 carcinoma cells

Sandip Patel, Manoranjan Santra, David J. McQuillan, Renato V. Iozzo, Andrew P. Thomas

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Several independent lines of evidence have implicated decorin, a small leucine-rich proteoglycan, in the inhibition of cell proliferation. However, the mechanism by which decorin mediates its effect on cell proliferation is unclear. Here we report, for the first time, decorinmediated increases in intracellular Ca2+ levels of single A431 cells. The effects of decorin persisted in the absence of extracellular Ca2+ but were blocked by AG1478, an epidermal growth factor (EGF)-specific tyrosine kinase inhibitor, and by down-regulation of the EGF receptor. The effects of decorin were not mimicked by the structurally homologous protein, biglycan. Our results indicate a novel action of decorin on the EGF receptor, which results in mobilization of intracellular Ca2+ providing a possible mechanism by which decorin causes growth suppression.

Original languageEnglish (US)
Pages (from-to)3121-3124
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number6
DOIs
StatePublished - Feb 6 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Decorin activates the epidermal growth factor receptor and elevates cytosolic Ca2+ in A431 carcinoma cells'. Together they form a unique fingerprint.

Cite this