Decreased heat shock response upon adipose differentiation of 3T3-L1 cells

Alice Y.C. Liu, Hueng Sik Choi, Myong Suk Bae-Lee

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

In order to gain a better understanding of the regulation of heat shock gene (hsp) expression in terminal cell differentiation, we evaluated the effects of heat shock on the synthesis of HSPs, the abundance of mRNAhsP, and the heat shock transcription factor (HSTF) DNA-binding activity in the 3T3-L1 fibroblasts and adipocytes. We showed that the heat shock (42°C) induction of synthesis of HSPs was significantly greater in the undifferentiated fibroblast than the differentiated adipocyte cultures. In particular, the heat shock induced synthesis of HSP 72 was at least 10 times greater in the fibroblasts than in the adipocytes. Analysis of mRNA of hsp 89α, hsp 89β, hsp 70, and hsp 25 by Northern blot hybridization showed that the expression of these mRNAs was very, if not strictly, dependent on heat shock of the cells; the abundance of these heat inducible mRNAs was significantly higher in fibroblasts than in adipocytes. Quantitation of the HSTF DNA-binding activity by gel retardation assay demonstrated a specific decrease in this activity in the differentiated cells. These results provide evidence of a decreased transcriptional activation of heat shock genes upon adipose cell differentiation.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number1
DOIs
StatePublished - Oct 15 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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