Deficient DNA binding of an apurinic/apyrimidinic DNA endonuclease activity from xeroderma pigmentosum cells

Lynn K. Bickley, Muriel W. Lambert

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A chromatin-associated apurinic/apyrimidinic (AP) DNA endonuclease activity, pI 9.8, from both normal human and xeroderma pigmentosum, complementation group A (XPA), lymphoblastoid cells was examined for its ability to bind AP DNA using a filter binding assay. The endonuclease activity from normal cells produced significantly greater binding to AP DNA than to untreated DNA, but this increase in binding was not observed when the XPA endonuclease was incubated with AP DNA versus untreated DNA. These results indicate that the XPA AP endonuclease activity is deficient in its ability to bind to AP DNA.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalCell Biology International Reports
Volume12
Issue number3
DOIs
StatePublished - Mar 1988

All Science Journal Classification (ASJC) codes

  • Cell Biology

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