Degradation of fibrillar forms of Alzheimer's amyloid β-peptide by macrophages

Amitabha Majumdar, Haeyong Chung, Georgia Dolios, Rong Wang, Nikiya Asamoah, Peter Lobel, Frederick R. Maxfield

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Cultured microglia internalize fibrillar amyloid Aβ (fAβ) and deliver it to lysosomes. Degradation of fAβ by microglia is incomplete, but macrophages degrade fAβ efficiently. When mannose-6 phosphorylated lysosomal enzymes were added to the culture medium of microglia, degradation of fAβ was increased, and the increased degradation was inhibited by excess mannose-6-phosphate, which competes for binding and endocytic uptake. This suggests that low activity of one or more lysosomal enzymes in the microglia was responsible for the poor degradation of fAβ. To further characterize the degradation of fAβ in late endosomes and lysosomes, we analyzed fAβ-derived intracellular degradation products in macrophages and microglia by mass spectrometry. Fragments with truncations in the first 12 N-terminal residues were observed in extracts from both cell types. We also analyzed material released by the cells. Microglia released mainly intact Aβ1-42, whereas macrophages released a variety of N-terminal truncated fragments. These results indicate that initial proteolysis near the N-terminus is similar in both cell types, but microglia are limited in their ability to make further cuts in the fAβ.

Original languageEnglish (US)
Pages (from-to)707-715
Number of pages9
JournalNeurobiology of Aging
Volume29
Issue number5
DOIs
StatePublished - May 2008

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

Keywords

  • Alzheimer's disease
  • Clearance
  • Macrophages
  • Mass spectrometry

Fingerprint Dive into the research topics of 'Degradation of fibrillar forms of Alzheimer's amyloid β-peptide by macrophages'. Together they form a unique fingerprint.

Cite this