Depolymerization of F-actin by deoxyribonuclease I

Sarah E. Hitchcock, Lars Carlsson, Uno Lindberg

Research output: Contribution to journalArticlepeer-review

208 Scopus citations

Abstract

Deoxyribonuclease I causes depolymerization of filamentous muscle actin to form a stable complex of 1 mole DNAase I:1 mole actin. The regulatory proteins tropomyosin and troponin bind to filamentous actin and slow down but do not prevent the depolymerization. In the absence of ATP, heavy meromyosin binds tightly to actin filaments and blocks completely the DNAase I:actin filament interaction. Addition of ATP releases heavy meromyosin; DNAase I is then rapidly inhibited and the actin filaments are depolymerized.

Original languageEnglish (US)
Pages (from-to)531-542
Number of pages12
JournalCell
Volume7
Issue number4
DOIs
StatePublished - Apr 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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