Depurination of ribosomal RNA and inhibition of viral RNA translation by an antiviral protein of Celosia cristata

V. K. Baranwal, Nilgun E. Tumer, H. C. Kapoor

Research output: Contribution to journalComment/debatepeer-review

13 Scopus citations

Abstract

An antiviral protein (25 kD) isolated from leaves of Celosia cristata (CCP 25) was tested for depurination study on ribosomal RNA from yeast. Ribosomal RNA yielded 360 nucleotide base fragment after treatment with CCP 25 indicating that CCP 25 was a ribosome inactivating protein. CCP 25 also inhibited translation of brome mosaic virus (BMV) and pokeweed mosaic virus (PMV) RNAs in rabbit reticulocyte translation system. The radioactive assay showed that incorporation of [35S]-methionine was less in translation proteins of BMV nucleic acid when CCP 25 was added to translation system. This indicated that antiviral protein from Celosia cristata not only depurinated ribosomal RNA but also inhibited translation of viral RNA in vitro.

Original languageEnglish (US)
Pages (from-to)1195-1197
Number of pages3
JournalIndian Journal of Experimental Biology
Volume40
Issue number10
StatePublished - Oct 1 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Cell Biology

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