Description of two novel members of the family erysipelotrichaceae: Ileibacterium valens gen. nov., sp. nov. and Dubosiella newyorkensis, gen. nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibacterium rodentium

Laura M. Cox, Jiho Sohn, Kerin L. Tyrrell, Diane M. Citron, Paul A. Lawson, Nisha B. Pate, Tadasu Iizumi, Guillermo I. Perez-Perez, Ellie J.C. Goldstein, Martin J. Blaser

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoidshaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7%. A second group, represented by NYU-BL-A4T, was most closely related to Faecalibaculum rodentium, with 86.6% 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9%) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10%) cellular fatty acids of strain NYU-BL-A3T were C16: 0 and C18: 0, and those of strain NYU-BL-A4T were C10: 0, C16: 0, C18: 0 and C18: 1ω9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4T being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3T (=ATCC TSD-63T=DSM 103668T) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4T (=ATCC TSD-64T=DSM 103457T) as the type strain.

Original languageEnglish (US)
Article number001793
Pages (from-to)1247-1254
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number5
DOIs
StatePublished - May 2017

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Keywords

  • Anaerobe
  • Dubosiella newyorkensis
  • Erysipelotrichaceae
  • Ileibacterium valens

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