Detection of DNA adducts by bioluminescence

Shunqing Xu, Xianglin Tan, Qunfeng Yao, Min He, Yikai Zhou, Jian Chen

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


Luminescent assay for detection ATP is very sensitive with limitation of 10-17 moles. ATP Using styrene oxide as a model carcinogen we currently apply a luminescence technique to detect the very low levles of carcinogen-DNA adducts in vitro and in vivo. The bioluminescent assay of DNA adducts entails three consecutive steps: digestion of modified DNA to adducted dinucleoside monophsphate(*NpN) and normal nucleotide are hydrolyzed to nucleosides(N) by nuclease P1 and prostatic acid phosphomonesterase(PAP); incorporation of γ -P into *NpN from ATP catalyzed by polynucleotide kinase, while no incorporation of γ -P of ATP into normal nucleoside(N); detection of consumption of ATP by luminescence. This assay does not require separate manipulation because of the selective property of nuclease P1. One fmol of carcinogen-DNA adducts was detected by luminescent assay. A good correlation between results of luminescent assy and 32P-postlabeling procedures has been observed. We detect 1 adduct in 108 nucleotides for 20 μg DNA sample. The procedures of luminescent method is very simple and low-cost. It appears applicable to the ultrasensitive detection of low levels of DNA adducts without radioactive isotope.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2001
Externally publishedYes
EventInternational Conference on Sensor Technology (ISTC 2001) - Wuhan, China
Duration: Oct 10 2001Oct 12 2001

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


  • Bioluminescence
  • Carcinogens
  • DNA adduct


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