Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels

L. D. Beckman, G. D. Frenkel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A procedure is described for the detection of DNA dependent DNA and RNA polymerase activities in intact polyacrylamide gels that contain DNA. After electrophoresis under non denaturing conditions, the intact gels are incubated with DNA or RNA polymerase reaction mixture in which one of the four deoxyribonucleoside or ribonucleoside triphosphates is radioactively labeled. The acid insoluble radioactivity associated with the intact gel is then analyzed by autoradiography of the intact gel or by liquid scintillation spectrometry of the sliced gel. Inhibition of the enzymatic activities by low molecular weight compounds such as N ethylmaleimide or rifampin can be demonstrated by this procedure.

Original languageEnglish (US)
Pages (from-to)1727-1737
Number of pages11
JournalNucleic acids research
Volume3
Issue number7
StatePublished - Dec 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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