Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels

L. D. Beckman, Gerald Frenkel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A procedure is described for the detection of DNA dependent DNA and RNA polymerase activities in intact polyacrylamide gels that contain DNA. After electrophoresis under non denaturing conditions, the intact gels are incubated with DNA or RNA polymerase reaction mixture in which one of the four deoxyribonucleoside or ribonucleoside triphosphates is radioactively labeled. The acid insoluble radioactivity associated with the intact gel is then analyzed by autoradiography of the intact gel or by liquid scintillation spectrometry of the sliced gel. Inhibition of the enzymatic activities by low molecular weight compounds such as N ethylmaleimide or rifampin can be demonstrated by this procedure.

Original languageEnglish (US)
Pages (from-to)1727-1737
Number of pages11
JournalNucleic Acids Research
Volume3
Issue number7
StatePublished - Dec 1 1976
Externally publishedYes

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DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Polyacrylamide Gel Electrophoresis
Gels
Deoxyribonucleosides
Ribonucleosides
Ethylmaleimide
Rifampin
Autoradiography
Radioactivity
Electrophoresis
Spectrum Analysis
Molecular Weight
Acids
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels. / Beckman, L. D.; Frenkel, Gerald.

In: Nucleic Acids Research, Vol. 3, No. 7, 01.12.1976, p. 1727-1737.

Research output: Contribution to journalArticle

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