Detection of Early Secretory Antigenic Target-6 Antibody for Diagnosis of Tuberculosis in Non-human Primates

Ganga V. Kanaujia, Manuel A. Garcia, Donna M. Bouley, Robert Peters, Maria Laura Gennaro

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Tuberculosis is one of the most economically devastating, zoonotic infections of captive non-human primates. The limitations of the tuberculin skin test, which is currently used to diagnose tuberculosis in living non-human primates, make it necessary to find new, simple, and economical diagnostic methods. We describe use of an enzyme-linked immunoassay to detect IgG antibodies against early secretory antigenic target (ESAT)-6, a small protein secreted by virulent tubercle bacilli, in paired (pre- and post-outbreak) sera from 57 non-human primates involved in an outbreak of Mycobacterium bovis infection in a research colony. Of 25 animals with tuberculosis lesions at necropsy, 22 (88%) had high serum levels of the ESAT-6 antibody. The ESAT-6 antibody was found in 16% (5/32) of post-outbreak sera from animals in which tuberculosis could not be confirmed at necropsy. The strong association between the ESAT-6 antibody and tuberculosis in non-human primates documented in this study, together with the robustness of the serologic assay, make the ESAT-6 ELISA a valuable tool for diagnosis of tuberculosis in captive non-human primates.

Original languageEnglish (US)
Pages (from-to)602-606
Number of pages5
JournalComparative Medicine
Volume53
Issue number6
StatePublished - Dec 1 2003

Fingerprint

tuberculosis
Primates
Tuberculosis
antibodies
Disease Outbreaks
Antibodies
Animals
necropsy
Serum
Tuberculin
Bacilli
Tuberculin Test
Mycobacterium Infections
tuberculin
skin tests
Mycobacterium bovis
Zoonoses
Skin Tests
Assays
immunoassays

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Kanaujia, Ganga V. ; Garcia, Manuel A. ; Bouley, Donna M. ; Peters, Robert ; Gennaro, Maria Laura. / Detection of Early Secretory Antigenic Target-6 Antibody for Diagnosis of Tuberculosis in Non-human Primates. In: Comparative Medicine. 2003 ; Vol. 53, No. 6. pp. 602-606.
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Detection of Early Secretory Antigenic Target-6 Antibody for Diagnosis of Tuberculosis in Non-human Primates. / Kanaujia, Ganga V.; Garcia, Manuel A.; Bouley, Donna M.; Peters, Robert; Gennaro, Maria Laura.

In: Comparative Medicine, Vol. 53, No. 6, 01.12.2003, p. 602-606.

Research output: Contribution to journalArticle

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