Autoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect (32)P- or (35)S-labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell. Both procedures are described here.
|Original language||English (US)|
|Pages (from-to)||Unit 14.4|
|Journal||Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]|
|State||Published - May 2001|
All Science Journal Classification (ASJC) codes
- Molecular Biology