Detection of hybridized probe.

Research output: Contribution to journalArticle

Abstract

Autoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect (32)P- or (35)S-labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell. Both procedures are described here.

Original languageEnglish (US)
Pages (from-to)Unit 14.4
JournalCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
VolumeChapter 14
StatePublished - May 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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