Detection of Listeria monocytogenes by using the polymerase chain reaction

M. T. Bessesen; Q. Luo; H. A. Rotbart; M. J. Blaser; R. T. Ellison

doi:10.1128/aem.56.9.2930-2932.1990

Detection of Listeria monocytogenes by using the polymerase chain reaction

M. T. Bessesen, Q. Luo, H. A. Rotbart, M. J. Blaser, R. T. Ellison

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a ³²P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strain, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

Original language	English (US)
Pages (from-to)	2930-2932
Number of pages	3
Journal	Applied and environmental microbiology
Volume	56
Issue number	9
DOIs	https://doi.org/10.1128/aem.56.9.2930-2932.1990
State	Published - 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.56.9.2930-2932.1990

Cite this

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title = "Detection of Listeria monocytogenes by using the polymerase chain reaction",

abstract = "A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strain, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.",

author = "Bessesen, {M. T.} and Q. Luo and Rotbart, {H. A.} and Blaser, {M. J.} and Ellison, {R. T.}",

year = "1990",

doi = "10.1128/aem.56.9.2930-2932.1990",

language = "English (US)",

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AU - Bessesen, M. T.

AU - Luo, Q.

AU - Rotbart, H. A.

AU - Blaser, M. J.

AU - Ellison, R. T.

PY - 1990

Y1 - 1990

N2 - A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strain, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

AB - A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strain, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

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U2 - 10.1128/aem.56.9.2930-2932.1990

DO - 10.1128/aem.56.9.2930-2932.1990

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AN - SCOPUS:0025050984

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SP - 2930

EP - 2932

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

IS - 9

ER -

Detection of Listeria monocytogenes by using the polymerase chain reaction

Abstract

All Science Journal Classification (ASJC) codes

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