Detection of tumor activated bust (TAB) antigen on an in vitro SJL/J B cell lymphoma line (cRCS-XHW) and its relationship to viral antigens

Howard J. Waichman, Nicholas M. Ponzio

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Abstract

B cell lymphomas (RCS) which arise in 90% of SJL/J mice at about 13 months of age are dependent on a host T cell response for growth in vivo. RCS cells express a superantigen (M/v-RCS) which stimulates syngeneic Vβ16+ T cells to produce tumor growth-promoting cytokines. Syngeneic T cells, RCS responsive and l-A'-responsive T-T hybrids, activated by 3-4 day exposure to some RCS cell lines were found to express unique antigenic determinants. mAbs raised against these determinants react with proteins between 150-220kd (non-reduced) and 75-97kd reduced. TAB determinants are not present on resting or on T cells activated by other stimuli. An in vitro RCS cell line [cRCS-XHW] was derived from a transplanted RCS tumor which induces TAB antigen expression on naive SJL/J T cells. Flow cytometry and western blot analysis revealed that cRCS-XHW cells also express TAB antigens. Further analysis of this TAB-inducing tumor revealed infection with Lactate Dehydrogenase-elevating Virus (LDV). Polyclonal anti-LDV antiserum stained 25-95% of cRCS-XHW, 40-95% of TAB cells, but other in vitro RCS lines were negative. RCS responsive as well as l-A+-responsive T-T hybrids stimulated with LDV+ RCS stained TAB+ while non RCS-responsive T-T hybrids failed to express these antigens. Using TAB and LDV specific antibodies, western blot analysis of TAB and RCS activated T-T hybrid total cell extracts indicates that TAB antiaens mav be LDV related oroteins since, both antibodies aooear to recognize identical protein bands.

Original languageEnglish (US)
Pages (from-to)A1466
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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