Determinants for Escherichia coli RNA polymerase assembly within the β subunit

Yi Wang, Konstantin Severinov, Nick Loizos, David Fenyö, Ewa Heyduk, Tomasz Heyduk, Brian T. Chait, Seth A. Darst

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We used binding assays and other approaches to identify fragments of the Escherichia coli RNAP β subunit involved in the obligatory interaction with the α subunit to form the stable assembly intermediate α2β as well as in the interaction to recruit the β' subunit into the α2β sub-assembly. We show that two regions of evolutionarily conserved sequence near the C terminus of β (conserved regions H and I) are central to the assembly of RNAP and likely make subunit-subunit contacts with both α and β'.

Original languageEnglish (US)
Pages (from-to)648-662
Number of pages15
JournalJournal of molecular biology
Volume270
Issue number5
DOIs
StatePublished - Aug 1 1997
Externally publishedYes

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Conserved Sequence
DNA-Directed RNA Polymerases
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Wang, Yi ; Severinov, Konstantin ; Loizos, Nick ; Fenyö, David ; Heyduk, Ewa ; Heyduk, Tomasz ; Chait, Brian T. ; Darst, Seth A. / Determinants for Escherichia coli RNA polymerase assembly within the β subunit. In: Journal of molecular biology. 1997 ; Vol. 270, No. 5. pp. 648-662.
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Wang, Y, Severinov, K, Loizos, N, Fenyö, D, Heyduk, E, Heyduk, T, Chait, BT & Darst, SA 1997, 'Determinants for Escherichia coli RNA polymerase assembly within the β subunit', Journal of molecular biology, vol. 270, no. 5, pp. 648-662. https://doi.org/10.1006/jmbi.1997.1139

Determinants for Escherichia coli RNA polymerase assembly within the β subunit. / Wang, Yi; Severinov, Konstantin; Loizos, Nick; Fenyö, David; Heyduk, Ewa; Heyduk, Tomasz; Chait, Brian T.; Darst, Seth A.

In: Journal of molecular biology, Vol. 270, No. 5, 01.08.1997, p. 648-662.

Research output: Contribution to journalArticle

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AU - Wang, Yi

AU - Severinov, Konstantin

AU - Loizos, Nick

AU - Fenyö, David

AU - Heyduk, Ewa

AU - Heyduk, Tomasz

AU - Chait, Brian T.

AU - Darst, Seth A.

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