Determinants for Escherichia coli RNA polymerase assembly within the β subunit

Yi Wang, Konstantin Severinov, Nick Loizos, David Fenyö, Ewa Heyduk, Tomasz Heyduk, Brian T. Chait, Seth A. Darst

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32 Scopus citations

Abstract

We used binding assays and other approaches to identify fragments of the Escherichia coli RNAP β subunit involved in the obligatory interaction with the α subunit to form the stable assembly intermediate α2β as well as in the interaction to recruit the β' subunit into the α2β sub-assembly. We show that two regions of evolutionarily conserved sequence near the C terminus of β (conserved regions H and I) are central to the assembly of RNAP and likely make subunit-subunit contacts with both α and β'.

Original languageEnglish (US)
Pages (from-to)648-662
Number of pages15
JournalJournal of molecular biology
Volume270
Issue number5
DOIs
StatePublished - Aug 1 1997

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • E. coli RNA polymerase assembly
  • Limited proteolysis
  • Protein-protein footprinting
  • Transcription

Cite this

Wang, Y., Severinov, K., Loizos, N., Fenyö, D., Heyduk, E., Heyduk, T., Chait, B. T., & Darst, S. A. (1997). Determinants for Escherichia coli RNA polymerase assembly within the β subunit. Journal of molecular biology, 270(5), 648-662. https://doi.org/10.1006/jmbi.1997.1139