Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N. Dickgiesser, B. N. Kreiswirth

Research output: Contribution to journalArticle

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Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

Original languageEnglish (US)
Pages (from-to)930-932
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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