Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N. Dickgiesser, B. N. Kreiswirth

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

Original languageEnglish (US)
Pages (from-to)930-932
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Kanamycin Kinase
Aminoglycosides
Staphylococcus aureus
DNA
Southern Blotting
Genes
Plasmids
aminocyclitol adenylyltransferase
aminoglycoside acetyltransferase

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

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Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization. / Dickgiesser, N.; Kreiswirth, B. N.

In: Antimicrobial agents and chemotherapy, Vol. 29, No. 5, 01.01.1986, p. 930-932.

Research output: Contribution to journalArticle

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AU - Dickgiesser, N.

AU - Kreiswirth, B. N.

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