Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

Zhihua Liu, Shengmin Sang, Thomas G. Hartman, Chi Tang Ho, Robert T. Rosen

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-3-heptanone, 5-methoxy-7-(4″-hydroxy-3″- methoxyphenyl)-1-phenyl-3-heptanone, 7-(4″-hydroxyphenyl) -1-phenylhept-4-en-3-one, 7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.

Original languageEnglish (US)
Pages (from-to)252-256
Number of pages5
JournalPhytochemical Analysis
Volume16
Issue number4
DOIs
StatePublished - Jul 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Keywords

  • Alpinia officinarum
  • Diarylheptanoids
  • HPLC-ECD
  • HPLC-PAD
  • Lesser galangal
  • Zingiberaceae

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