Development and characterization of 12 microsatellite loci from the blueberry gall midge Dasineura oxycoccana (Diptera: Cecidomyiidae)

Hyojoong Kim, Cesar Rodriguez-Saona, Deok Ho Kwon, Sangwook Park, Taek Jun Kang, Se Jin Kim, Ki Jeong Hong, Heung Sik Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The blueberry gall midge, Dasineura oxycoccana (Johnson), is a serious insect pest of blueberries and cranberries in North America. In this study, we developed 12 novel microsatellite loci for this gall midge. To obtain its sequence data, next-generation sequencing was performed using mixed individuals collected from Korea and the USA. The developed loci were polymorphic, with four to nine alleles in 20 individuals from a single population of New Jersey, USA. The analyses revealed that all 20 individuals had different multilocus genotypes with expected heterozygosity ranging from 0.708 to 0.920. In addition, we confirmed the discriminative resolution of all individuals collected. These markers will facilitate future studies on the genetic structure of blueberry gall midge populations.

Original languageEnglish (US)
Pages (from-to)415-418
Number of pages4
JournalApplied Entomology and Zoology
Volume50
Issue number3
DOIs
StatePublished - Aug 18 2015

All Science Journal Classification (ASJC) codes

  • Insect Science

Keywords

  • Biological invasion
  • Exotic insect pest
  • Microsatellite
  • Population genetics
  • SSR marker

Fingerprint Dive into the research topics of 'Development and characterization of 12 microsatellite loci from the blueberry gall midge Dasineura oxycoccana (Diptera: Cecidomyiidae)'. Together they form a unique fingerprint.

Cite this