Development and in-vitro evaluation of co-loaded berberine chloride and evodiamine ethosomes for treatment of melanoma

Hongmei Lin, Longfei Lin, Yoonsun Choi, Bozena Michniak-Kohn

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Berberine chloride (BBR) and evodiamine (EVO) are two main active ingredients of “ZuoJinWan”, a classical Chinese herbal medicine, and these compounds are known to have a synergistic inhibitory effect on various cancer cell lines. Several recent studies have reported anti-melanoma effects for both BBR and EVO. However, topical delivery of the two compounds has been challenging, due to their poor aqueous solubility and their low skin penetration. In the current study, we have combined BBR and EVO into an ethosomes delivery system with the future aim to design a novel topical anti-melanoma formulation. The ethosomes formulations were characterized using particle size, entrapment efficiency and an in vitro skin drug deposition study. The ethosome formulation displaying maximum drug deposition in the epidermis was selected for further study. This formulation contained ethosomes with mean size of 171 nm and 90% or above entrapment efficiency for both BBR and EVO. Cell viability tests proved the optimized ethosomes increased the inhibitory effect on B16 melanoma cells. These results corroborate that ethosomes containing a combination of BBR and EVO are a promising delivery system for potential use in melanoma therapy.

Original languageEnglish (US)
Article number119278
JournalInternational Journal of Pharmaceutics
Volume581
DOIs
StatePublished - May 15 2020

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Keywords

  • Berberine chloride
  • Ethosomes
  • Evodiamine
  • Melanoma
  • Topical delivery

Fingerprint

Dive into the research topics of 'Development and in-vitro evaluation of co-loaded berberine chloride and evodiamine ethosomes for treatment of melanoma'. Together they form a unique fingerprint.

Cite this