Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls

Martin Nygren, Marianne Hansson, Michael Sjöström, Christoffer Rappe, Peter Kahn, Michael Gochfeld, Henry Velez, Therese Ghent-Guenther, Wayne P. Wilson

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

A method which allows measurements of low levels (fg/g) of PCDDs and PCDFs in blood plasma, has been developed and validated at four fortified levels between 0.027 ppt and 0.9 ppt. The procedure involves extraction with organic solvents, multiple cleanup using various absorbents and finally detection and quantification by multiple-ion-mode high resolution gas chromato graphy/high resolution mass spectrometry. This method has been found to have a limit of detection of 0.003 to 0.02 ppt depending on the isomer studied. Using this method, samples of blood plasma have been analyzed for PCDDs and PCDFs from Viet Nam veterans from The United States, and from matched controls, and have been compared with the results of the analyses of adipose tissue. Exposed Vietnam Veterans could be separated from matched controls using a multivariate data analysis.

Original languageEnglish (US)
Pages (from-to)1663-1692
Number of pages30
JournalChemosphere
Volume17
Issue number9
DOIs
StatePublished - 1988

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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