Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls

Martin Nygren; Marianne Hansson; Michael Sjöström; Christoffer Rappe; Peter Kahn; Michael Gochfeld; Henry Velez; Therese Ghent-Guenther; Wayne P. Wilson

doi:10.1016/0045-6535(88)90095-1

Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls

Martin Nygren, Marianne Hansson, Michael Sjöström, Christoffer Rappe, Peter Kahn, Michael Gochfeld, Henry Velez, Therese Ghent-Guenther, Wayne P. Wilson

School of Environmental and Biological Sciences, Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A method which allows measurements of low levels (fg/g) of PCDDs and PCDFs in blood plasma, has been developed and validated at four fortified levels between 0.027 ppt and 0.9 ppt. The procedure involves extraction with organic solvents, multiple cleanup using various absorbents and finally detection and quantification by multiple-ion-mode high resolution gas chromato graphy/high resolution mass spectrometry. This method has been found to have a limit of detection of 0.003 to 0.02 ppt depending on the isomer studied. Using this method, samples of blood plasma have been analyzed for PCDDs and PCDFs from Viet Nam veterans from The United States, and from matched controls, and have been compared with the results of the analyses of adipose tissue. Exposed Vietnam Veterans could be separated from matched controls using a multivariate data analysis.

Original language	English (US)
Pages (from-to)	1663-1692
Number of pages	30
Journal	Chemosphere
Volume	17
Issue number	9
DOIs	https://doi.org/10.1016/0045-6535(88)90095-1
State	Published - 1988

All Science Journal Classification (ASJC) codes

Environmental Engineering
Environmental Chemistry
Chemistry(all)
Pollution
Health, Toxicology and Mutagenesis

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Nygren, M., Hansson, M., Sjöström, M., Rappe, C., Kahn, P., Gochfeld, M., Velez, H., Ghent-Guenther, T., & Wilson, W. P. (1988). Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls. Chemosphere, 17(9), 1663-1692. https://doi.org/10.1016/0045-6535(88)90095-1

Nygren, Martin ; Hansson, Marianne ; Sjöström, Michael ; Rappe, Christoffer ; Kahn, Peter ; Gochfeld, Michael ; Velez, Henry ; Ghent-Guenther, Therese ; Wilson, Wayne P. / Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls. In: Chemosphere. 1988 ; Vol. 17, No. 9. pp. 1663-1692.

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title = "Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls",

abstract = "A method which allows measurements of low levels (fg/g) of PCDDs and PCDFs in blood plasma, has been developed and validated at four fortified levels between 0.027 ppt and 0.9 ppt. The procedure involves extraction with organic solvents, multiple cleanup using various absorbents and finally detection and quantification by multiple-ion-mode high resolution gas chromato graphy/high resolution mass spectrometry. This method has been found to have a limit of detection of 0.003 to 0.02 ppt depending on the isomer studied. Using this method, samples of blood plasma have been analyzed for PCDDs and PCDFs from Viet Nam veterans from The United States, and from matched controls, and have been compared with the results of the analyses of adipose tissue. Exposed Vietnam Veterans could be separated from matched controls using a multivariate data analysis.",

author = "Martin Nygren and Marianne Hansson and Michael Sj{\"o}str{\"o}m and Christoffer Rappe and Peter Kahn and Michael Gochfeld and Henry Velez and Therese Ghent-Guenther and Wilson, {Wayne P.}",

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Nygren, M, Hansson, M, Sjöström, M, Rappe, C, Kahn, P, Gochfeld, M, Velez, H, Ghent-Guenther, T & Wilson, WP 1988, 'Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls', Chemosphere, vol. 17, no. 9, pp. 1663-1692. https://doi.org/10.1016/0045-6535(88)90095-1

Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls. / Nygren, Martin; Hansson, Marianne; Sjöström, Michael; Rappe, Christoffer; Kahn, Peter; Gochfeld, Michael; Velez, Henry; Ghent-Guenther, Therese; Wilson, Wayne P.

In: Chemosphere, Vol. 17, No. 9, 1988, p. 1663-1692.

Research output: Contribution to journal › Article › peer-review

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T1 - Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls

AU - Nygren, Martin

AU - Hansson, Marianne

AU - Sjöström, Michael

AU - Rappe, Christoffer

AU - Kahn, Peter

AU - Gochfeld, Michael

AU - Velez, Henry

AU - Ghent-Guenther, Therese

AU - Wilson, Wayne P.

PY - 1988

Y1 - 1988

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AB - A method which allows measurements of low levels (fg/g) of PCDDs and PCDFs in blood plasma, has been developed and validated at four fortified levels between 0.027 ppt and 0.9 ppt. The procedure involves extraction with organic solvents, multiple cleanup using various absorbents and finally detection and quantification by multiple-ion-mode high resolution gas chromato graphy/high resolution mass spectrometry. This method has been found to have a limit of detection of 0.003 to 0.02 ppt depending on the isomer studied. Using this method, samples of blood plasma have been analyzed for PCDDs and PCDFs from Viet Nam veterans from The United States, and from matched controls, and have been compared with the results of the analyses of adipose tissue. Exposed Vietnam Veterans could be separated from matched controls using a multivariate data analysis.

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