Development of a high-throughput micro-neurocircuitry platform for drug screening studies

Joseph A. Fantuzzo, Vincent R. Mirabella, Ronald P. Hart, Zhiping P. Pang, Jeffrey D. Zahn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Models for studying the onset and development of neuropsychiatric disorders have generally been limited to single or co-cultured mixed cell populations in vitro or animal models. While these approaches shed light on important disease mechanisms, they either do not capture the complex circuitry of the brain or limit translation of findings due to species differences. Here, we have used induced neurons (iNs) derived from patient-specific induced pluripotent stem cells within an arrayed microdevice which compartmentalizes iNs into two chambers. We demonstrate that we can form a functional circuit, which can be perturbed by various small molecules.

Original languageEnglish (US)
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages287-290
Number of pages4
ISBN (Electronic)9781510897571
StatePublished - Jan 1 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume1

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period11/11/1811/15/18

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

Keywords

  • Calcium imaging
  • Compartmentalization
  • DREADDs
  • High-throughput
  • Induced neurons
  • Microfluidics
  • Stem cells

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