Development of a highly specific and sensitive molecular probe for detection of cutaneous lymphoma

Matthias Volkenandt, H. Peter Soyer, Helmut Kerl, Joseph R. Bertino

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Using DNA from formalin-fixed paraffin-embedded tissue of a patient with mycosis fungoides, a highly specific and sensitive molecular probe for the malignant lymphoid cells of this patient was developed. Polymerase chain reaction (PCR) was used to selectively amplify the region of the rearrangement of TCR-γ genes. Randomly inserted nucleotides between rearranged segments (N-region) were used as a specific marker for the malignant lymphoid clone of this patient. Clonal cells in paraffin-embedded tissue of previous and later biopsies were detected. This technique provides a new and unique tool for retrospective detection of early evolving cutaneous lymphoma, as well as for detection of minimal residual and systemic disease.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalJournal of Investigative Dermatology
Volume97
Issue number1
DOIs
StatePublished - Jun 1991

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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