Development of a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for the Inhibition of Keap1–Nrf2 Protein–Protein Interaction

Keyphrases

• Protein-protein Interaction 100%
• Single-molecule Fluorescence Resonance Energy Transfer (smFRET) 100%
• Fluorescence Resonance Energy Transfer Assay 100%
• Homogeneous Time-resolved Fluorescence 100%
• Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) 66%
• Small Molecule Inhibitors 33%
• Transcription Factor 16%
• High-throughput Screening 16%
• Therapeutic Agents 16%
• Translocated 16%
• Response Element 16%
• Lead Optimization 16%
• Antioxidant System 16%
• Associated Proteins 16%
• Competition Assay 16%
• Oxidative Damage 16%
• 26S Proteasome 16%
• Potent Inhibitor 16%
• Fluorescent Probe 16%
• Preventive Agent 16%
• Peptide Amide 16%
• Cytoprotective Enzymes 16%
• Kelch-like ECH-associated Protein 1 (Keap1) 16%
• Kelch Domain 16%
• Fluorescence Polarization Assay 16%
• Antioxidant Enzymes 16%
• As(V) 16%
• Kelch-like 16%
• High Dynamic Range 16%
• Intracellular Level 16%
• Incubation Time 16%
• Assay Conditions 16%
• Inhibition Constant 16%
• Nrf2 Activator 16%
• Fluorescein Isothiocyanate 16%
• Screening Optimization 16%
• Z-factor 16%
• Subnanomolar Inhibition 16%
• Buffer Type 16%

Biochemistry, Genetics and Molecular Biology

• Fluorescence Resonance Energy Transfer 100%
• Transcription Factor Nrf2 100%
• Small Molecule 33%
• High Throughput Screening 16%
• Response Element 16%
• Transcription Factors 16%
• Proteasome 16%
• Fluorescent Probe 16%
• Kelch Motif 16%
• Antioxidant Enzyme 16%
• Incubation Time 16%
• Fluorescein Isothiocyanate 16%

Pharmacology, Toxicology and Pharmaceutical Science

• Transcription Factor Nrf2 100%
• Antioxidant 33%
• Transcription Factors 16%
• High-Throughput Screening 16%
• Fluorescent Dye 16%
• Cytoprotective Agent 16%
• Carboxamide 16%
• Proteasome 16%
• Buffer 16%
• Incubation Time 16%
• Fluorescein Isothiocyanate 16%
• Diseases 16%