Development of a ligand-directed approach to study the pathogenesis of invasive aspergillosis

Michail S. Lionakis, Johanna Lahdenranta, Jessica Sun, Wei Liu, Russell E. Lewis, Nathaniel D. Albert, Renata Pasqualini, Wadih Arap, Dimitrios P. Kontoyiannis

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Invasive aspergiliosis is a leading cause of infectious death in immunosuppressed patients. Here, we adapted a phage display library-based selection to screen and identify binding peptides to the surface of Aspergillus fumigatus conidia and hyphae. We identified a peptide (sequence CGGRLGPFC) that reliably binds to the surface of Aspergillus fumigatus hyphae. Binding was not Aspergillus strain specific, as it was also observed in hyphae of other Aspergillus clinical isolates. Furthermore, CGGRLGPFC-displaying phage targets Aspergillus fumigatus hyphae on formalin-fixed paraffin-embedded histopathology sections of lung tissue recovered from mice with invasive pulmonary aspergiliosis. This approach may yield reagents such as peptidomimetics for novel diagnostic and therapeutic interventions in invasive aspergiliosis.

Original languageEnglish (US)
Pages (from-to)7747-7758
Number of pages12
JournalInfection and immunity
Volume73
Issue number11
DOIs
StatePublished - Nov 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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