Development of a microfluidic module for DNA purification from bacterial lysates via phenol extraction

Mercedes C. Morales, Jeffrey D. Zahn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work microfluidic platforms designed to miniaturize the phenol extraction technique commonly used to purify DNA from cell lysates have been developed. First, a long serpentine device was used to demonstrate protein partitioning and DNA purification when both analytes were infused into the aqueous inlet. Next, a redesigned shorter device was fabricated to extract plasmid DNA from bacterial lysates using droplet based flow. The plasmid recovery found using the microdevice was high and comparable to the recovery achieved using commercial kits and standard phenol extraction.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages746-748
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period11/1/0911/5/09

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Keywords

  • DNA purification
  • Multi-phase microfluidics
  • Phenol extraction

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