Development of imaging techniques to study the pathogenesis of biosafety level 2/3 infectious agents

Courtney E. Rella, Nancy Ruel, Eliseo A. Eugenin

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Despite significant advances in microbiology and molecular biology over the last decades, several infectious diseases remain global concerns, resulting in the death of millions of people worldwide each year. According to the Center for Disease Control (CDC) in 2012, there were 34 million people infected with HIV, 8.7 million new cases of tuberculosis, 500 million cases of hepatitis, and 50-100 million people infected with dengue. Several of these pathogens, despite high incidence, do not have reliable clinical detection methods. New or improved protocols have been generated to enhance detection and quantitation of several pathogens using high-end microscopy (light, confocal, and STORM microscopy) and imaging software. In the current manuscript, we discuss these approaches and the theories behind these methodologies. Thus, advances in imaging techniques will open new possibilities to discover therapeutic interventions to reduce or eliminate the devastating consequences of infectious diseases. This manuscript describes the new imaging technology available for detection with unprecedented sensitivity of several pathogens.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalPathogens and disease
Volume72
Issue number3
DOIs
StatePublished - Dec 1 2014

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Keywords

  • Dengue
  • HIV
  • Tuberculosis
  • Viruses

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