Dietary fructose inhibits lactation-induced adaptations in rat 1,25-(OH)2D3 synthesis and calcium transport

Veronique Douard, Takuji Suzuki, Yves Sabbagh, Jacklyn Lee, Sue Shapses, Sheldon Lin, Ronaldo P. Ferraris

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We recently showed that excessive fructose consumption, already associated with numerous metabolic abnormalities, reduces rates of intestinal Ca 2+transport. Using a rat lactation model with increased Ca 2+requirements, we tested the hypothesis that mechanisms underlying these inhibitory effects of fructose involve reductions in renal synthesis of 1,25-(OH)2D3. Pregnant and virgin (control) rats were fed isocaloric fructose or, as controls, glucose, and starch diets fromd2of gestation to the end of lactation. Compared to virgins, lactating dams fed glucose or starch had higher rates of intestinal transcellular Ca2+ transport, elevated intestinal and renal expression of Ca2+ channels, Ca2+-binding proteins, and CaATPases, as well as increased levels of 25-(OH)D3 and 1,25-(OH)2D3. Fructose consumption prevented almost all of these lactation-induced increases, and reduced vitamin D receptor binding to promoter regions of Ca 2+channels and binding proteins. Changes in 1,25-(OH) 2D3level were tightly correlated with alterations in expression of 1α-hydroxylase but not with levels of parathyroid hormone and of 24-hydroxylase. Bone mineral density, content, and mechanical strength each decreased with lactation, but then fructose exacerbated these effects. When Ca2+ requirements increase during lactation or similar physiologically challenging conditions, excessive fructose consumption may perturb Ca2+ homeostasis because of fructose-induced reductions in synthesis of 1,25-(OH)2D3.

Original languageEnglish (US)
Pages (from-to)707-721
Number of pages15
JournalFASEB Journal
Issue number2
StatePublished - Feb 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


  • Bone
  • Intestine
  • Kidney
  • Parathyroid hormone
  • Vitamin D

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