Dietary phosphorus-responsive genes in the intestine, pyloric ceca, and kidney of rainbow trout

Shozo H. Sugiura, Ronaldo P. Ferraris

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Identification of phosphorus (P)-responsive genes is important in diagnosing the adequacy of dietary P intake well before clinical symptoms arise. The mRNA abundance of selected genes was determined in the intestine, pyloric ceca, and kidney of rainbow trout fed low-P (LP) or sufficient-P (SP) diet for 2, 5, and 20 days. The LP-to-SP ratio (LP/SP) of mRNA abundance was used to evaluate the difference in gene expression between LP and SP fish, and to compare the response with bone and serum P, which are conventional indicators of P status. The LP/SP of intestinal, cecal, and renal type II sodium-phosphate cotransporter (NaPi-II) mRNA abundance changed from ∼1-2 (day 2) to ∼1.4-4 (day 5) and to ∼2-10 (day 20). The LP/SP of renal NaPi-II, vitamin D 24-hydroxylase, and vitamin D receptor mRNA abundance correlated inversely with serum P on day 5 but not on day 2 and day 20. In another study, differentially expressed genes between LP and SP fish were examined by subtractive hybridization, confirmed by Northern blot, and evaluated by t-test and correlation with serum and bone P concentrations. About 30 genes were identified as dietary P responsive at day 20, including intestinal meprin and cysteinesulfinic acid decarboxylase, renal S 100 calcium-binding protein and mitochondrial Pi carrier, and cecal apolipoprotein E, somatomedin B-related protein, and NaPi-II. The LP/SP of mRNA abundance of renal mitochondrial P i carrier and intestinal cysteine-sulfinic acid decarboxylase changed significantly by day 2, and intestinal meprin by day 5. Hence, these genes and NaPi-II are among the earliest steady-response genes capable of predicting P deficiency well before the onset of clinical deficiency.

Original languageEnglish (US)
Pages (from-to)R541-R550
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume287
Issue number3 56-3
DOIs
StatePublished - Sep 2004

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Keywords

  • Dietary regulation
  • Gene expression
  • Hypophosphatemia
  • Sodium phosphate
  • Vitamin D

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