Differences between store-dependent Ca2+ fluxes in lymphocytes from African Americans and whites

Zoltan Fekete, Masayuki Kimura, Makoto Horiguchi, Jeffrey P. Gardner, Frederic Nash, Abraham Aviv

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Objective. To investigate differences between store-dependent Ca2+ in African American and white men. Method. Thapsigargin, a potent and specific inhibitor of the sarco(endo)plasmic reticulum Ca2+-ATPase, was used as a probe to elicit store-dependent Ca2+ fluxes. Treatment with this agent caused a rise in the cytosolic free Ca2+ due to the egress of Ca2+ from thapsigargin-sensitive Ca2+ stores and the acceleration of external Ca2+ influx through store-dependent Ca2+ channels. Design. Lymphocytes were obtained from 22 African Americans and 23 whites. These cells were subjected to thapsigargin treatment and changes in the cellular Ca2+ profiles were monitored. Results. Both in Ca2+-free and in Ca2+-containing media the increases in cytosolic free Ca2+ concentrations after thapsigargin treatment were greater in lymphocytes from African Americans than they were in those from whites. The greater levels of cytosolic Ca2+ concentration were coupled with higher rates of Ca2+ extrusion in thapsigargin-treated lymphocytes from African Americans. Conclusions. These findings suggest that store-dependent Ca2+ fluxes are greater in lymphocytes from African Americans than they are in those from whites. This phenomenon increases the Ca2+ turnover rate and might augment the sensitivity to agonists acting through Ca2+ signaling systems, thereby predisposing African Americans to essential hypertension.

Original languageEnglish (US)
Pages (from-to)1293-1299
Number of pages7
JournalJournal of Hypertension
Volume14
Issue number11
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Ca channels
  • Ca-ATPase
  • Endoplasmic reticulum
  • Essential
  • Ethnic groups
  • Hypertension

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