Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis

Riccardo Manganelli, Eugenie Dubnau, Sanjay Tyagi, Fred Russell Kramer, Issar Smith

Research output: Contribution to journalArticle

283 Scopus citations


The ability of Mycobacterium tuberculosis to adapt to different environments in the infected host is essential for its pathogenicity. Consequently, this organism must be able to modulate gene expression to respond to the changing conditions it encounters during infection. In this paper we begin a comprehensive study of M. tuberculosis gene regulation, characterizing the transcript levels of 10 of its 13 putative sigma factor genes. We developed a real-time RT-PCR assay using a family of novel fluorescent probes called molecular beacons to quantitatively measure the different mRNAs. Three sigma factor genes were identified that have increased mRNA levels after heat shock, two of which also responded to detergent stress. In addition, we also identified a sigma factor gene whose mRNA increased after mild cold shock and a second that responded to conditions of low aeration.

Original languageEnglish (US)
Pages (from-to)715-724
Number of pages10
JournalMolecular microbiology
Issue number2
StatePublished - Jan 30 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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