Using enhanced negative dielectrophoresis (nDEP), or ultra-DEP (uDEP), we demonstrate an electronic actuation system to perform a multiplexed bead-based protein assay (Figure 1). We pattern an array of proteins along a single microfluidic channel, where each element targets a specific secondary protein coated on micron-sized beads in the subsequently introduced sample. Below each element of the array, we have a pair of addressable interdigitated electrodes (IDE). By selectively applying voltage at the terminals of each IDE pair, the DEP force detaches protein-bound beads from each element of the array, one by one, without disturbing the bound beads in the neighboring regions. The detached beads can be quantified optically or electrically downstream. Here, we illustrate 16-plex actuation capability of our device to elute beads surface-bound through anti-IgG and IgG interaction which is on the same order of magnitude in strength as typical antibody-antigen interactions.