Digital assay for multiplexed detection of protein biomarkers

S. Emaminejad, J. Mok, M. Mindrinos, R. W. Davis, M. Javanmard

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using enhanced negative dielectrophoresis (nDEP), or ultra-DEP (uDEP), we demonstrate an electronic actuation system to perform a multiplexed bead-based protein assay (Figure 1)[1]. We pattern an array of proteins along a single microfluidic channel, where each element targets a specific secondary protein coated on micron-sized beads in the subsequently introduced sample. Below each element of the array, we have a pair of addressable interdigitated electrodes (IDE). By selectively applying voltage at the terminals of each IDE pair, the DEP force detaches protein-bound beads from each element of the array, one by one, without disturbing the bound beads in the neighboring regions. The detached beads can be quantified optically or electrically downstream. Here, we illustrate 16-plex actuation capability of our device to elute beads surface-bound through anti-IgG and IgG interaction which is on the same order of magnitude in strength as typical antibody-antigen interactions.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1086-1088
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Keywords

  • Dielectrophoresis
  • Digital proteomics
  • Microfluidics
  • Protein detection

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