Directing neurite growth in 3D collagen scaffolds with gradients of mechanical properties

Harini G. Sundararaghavan, David I. Shreiber

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomaterial scaffolds for nerve and spinal cord regeneration must not only promote neurite re-growth but also direct it. Several cell types, including neurons, respond to the mechanical properties of the substrate on which they are grown. We believe that gradients of mechanical properties can be used to direct neurons. To spatially control the mechanical properties, gradients of genipin - a naturally occurring, cell-tolerated crosslinking agent - are created in 3D through a compliant collagen gel using microfluidics. Gradients of mechanical properties are evaluated by measuring genipin-induced fluorescence, which we have previously correlated to mechanical properties. Growth of neurites was evaluated in gels of uniform stiffness and a gradient generated by incubation in 0 to 1 mM genipin for 12hrs to produce approximately an order of magnitude change in the shear modulus. Neurite growth was evaluated 5 days after gradient formation. Neurites demonstrated a directional bias against the gradient of stiffness. These results demonstrate that neurites can respond to subtle gradients of mechanical properties within a 3D scaffold and point to opportunities to manipulate properties for directed nerve and spinal cord regeneration.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages59-60
Number of pages2
ISBN (Print)0791847985, 9780791847985
DOIs
StatePublished - Jan 1 2007
Event2007 ASME Summer Bioengineering Conference, SBC 2007 - Keystone, CO, United States
Duration: Jun 20 2007Jun 24 2007

Publication series

NameProceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007

Other

Other2007 ASME Summer Bioengineering Conference, SBC 2007
CountryUnited States
CityKeystone, CO
Period6/20/076/24/07

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Directing neurite growth in 3D collagen scaffolds with gradients of mechanical properties'. Together they form a unique fingerprint.

  • Cite this

    Sundararaghavan, H. G., & Shreiber, D. I. (2007). Directing neurite growth in 3D collagen scaffolds with gradients of mechanical properties. In Proceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007 (pp. 59-60). (Proceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/sbc2007-176659