Distribution of H-2 alloantigen in adult and developing mouse brain

Melitta Schachner, Richard L. Sidman

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Quantitative assays for the major histocompatibility antigen system H-2 in mouse brain were carried out by absorption technique. The representation of H-2 in brain reaches the adult level 3-4 weeks after birth and is approximately equal in various parts of the brain. A method is described for obtaining single cell suspensions by trypsinization of developing mouse brain and the cells were used in a complement mediated cytotoxic test. H-2 is stable to trypsin treatment and was detected by this method at 8 days but not at 4 days after birth. While the direct cytotoxic test is less sensitive than the quantitative absorption methods, it should serve for detection of antigenic specificities unique to brain.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalBrain research
Volume60
Issue number1
DOIs
StatePublished - Sep 28 1973
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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