Abstract
Quantitative assays for the major histocompatibility antigen system H-2 in mouse brain were carried out by absorption technique. The representation of H-2 in brain reaches the adult level 3-4 weeks after birth and is approximately equal in various parts of the brain. A method is described for obtaining single cell suspensions by trypsinization of developing mouse brain and the cells were used in a complement mediated cytotoxic test. H-2 is stable to trypsin treatment and was detected by this method at 8 days but not at 4 days after birth. While the direct cytotoxic test is less sensitive than the quantitative absorption methods, it should serve for detection of antigenic specificities unique to brain.
Original language | English (US) |
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Pages (from-to) | 191-198 |
Number of pages | 8 |
Journal | Brain research |
Volume | 60 |
Issue number | 1 |
DOIs | |
State | Published - Sep 28 1973 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology