Diurnal and bathymetric changes in chlorophyll fluorescence yields of reef corals measured in situ with a fast repetition rate fluorometer

M. P. Lesser, M. Y. Gorbunov

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

A newly developed underwater fast repetition rate fluorometer (FRRF) was used for in situ measurements of chlorophyll fluorescence yields on the reef-building corals Montastraea faveolata and Montastraea cavernosa from around Lee Stocking Island, Bahamas. Diel studies of the quantum yield of chlorophyll fluorescence (ΔF′/Fm′) in photosystem II (PSII) reveal a pattern of mid-day depression of ΔF′/Fm′ in both of these species of coral. At the same time, non-photochemical quenching (qN) increased significantly during the day, a pattern consistent with the regulation of PSII by dynamic photoinhibition mediated by non-photochemical quenching. Despite these mid-day depressions in ΔF′/Fm′, net productivity, measured as oxygen flux, remains high, suggesting that non-photochemical quenching dissipates the majority of the absorbed photons at mid-day and protects the photosynthetic apparatus, allowing the endosymbiotic dinoflagellates (zooxanthellae) to operate at maximum rates of photosynthesis. In 1999 measurements of ΔF′/Fm′ on M. faveolata over a bathymetric range of 2 to 30 m showed an increase in ΔF′/Fm′ with increasing depth when measured at the same time of day. This suggests, although there is year-to-year variability, that changes in the underwater light field, and photoacclimation to that light field, control the degree of photoprotection attributable to non-photochemical quenching in the zooxanthellae of these corals. The fluorescence yields of M. faveolata exposed to elevated temperatures (>32°C) in the field showed a significant decrease in ΔF′/Fm′ before visible signs (e.g., paling of colonies) occurred. It was also possible to predict which colonies at the same depth and light regime would bleach first in response to elevated temperatures before any visible signs of bleaching were evident using ΔF′/Fm′ as a predictor.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalMarine Ecology Progress Series
Volume212
DOIs
StatePublished - Mar 8 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Keywords

  • Chlorophyll fluorescence
  • Coral
  • Coral bleaching
  • Quantum yield

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