DNA complementary to rabbit globin mRNA made by E. coli polymerase I

Mukund J. Modak; Stuart L. Marcus; Liebe F. Cavalieri

doi:10.1016/S0006-291X(73)80051-8

DNA complementary to rabbit globin mRNA made by E. coli polymerase I

Mukund J. Modak, Stuart L. Marcus, Liebe F. Cavalieri

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

E. coli DNA polymerase I has been used to synthesize DNA complementary to rabbit globin mRNA. In addition to the heteropolymeric DNA, poly (dT) and poly (dA)·(dT) are also synthesized. The extent of synthesis of these three products decreases at different rates upon heat inactivation of the polymerase, suggesting that the sites of synthesis on the enzyme are either entirely or partially separate. The use of polymerase I for copying RNAs makes possible the ready availability of complementary DNAs.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(73)80051-8
State	Published - Nov 1 1973
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "E. coli DNA polymerase I has been used to synthesize DNA complementary to rabbit globin mRNA. In addition to the heteropolymeric DNA, poly (dT) and poly (dA)·(dT) are also synthesized. The extent of synthesis of these three products decreases at different rates upon heat inactivation of the polymerase, suggesting that the sites of synthesis on the enzyme are either entirely or partially separate. The use of polymerase I for copying RNAs makes possible the ready availability of complementary DNAs.",

author = "Modak, {Mukund J.} and Marcus, {Stuart L.} and Cavalieri, {Liebe F.}",

note = "Funding Information: This work was supported in part by NCI Grant CA 08748 and AEC Contract AT (30-1) -910.",

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AU - Modak, Mukund J.

AU - Marcus, Stuart L.

AU - Cavalieri, Liebe F.

N1 - Funding Information: This work was supported in part by NCI Grant CA 08748 and AEC Contract AT (30-1) -910.

PY - 1973/11/1

Y1 - 1973/11/1

N2 - E. coli DNA polymerase I has been used to synthesize DNA complementary to rabbit globin mRNA. In addition to the heteropolymeric DNA, poly (dT) and poly (dA)·(dT) are also synthesized. The extent of synthesis of these three products decreases at different rates upon heat inactivation of the polymerase, suggesting that the sites of synthesis on the enzyme are either entirely or partially separate. The use of polymerase I for copying RNAs makes possible the ready availability of complementary DNAs.

AB - E. coli DNA polymerase I has been used to synthesize DNA complementary to rabbit globin mRNA. In addition to the heteropolymeric DNA, poly (dT) and poly (dA)·(dT) are also synthesized. The extent of synthesis of these three products decreases at different rates upon heat inactivation of the polymerase, suggesting that the sites of synthesis on the enzyme are either entirely or partially separate. The use of polymerase I for copying RNAs makes possible the ready availability of complementary DNAs.

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DNA complementary to rabbit globin mRNA made by E. coli polymerase I

Abstract

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