DNA interpolyelectrolyte complexes as a tool for efficient cell transformation

Alexander V. Kabanov, Irina V. Astafyeva, Mikhail L. Chikindas, Gennadii F. Rosenblat, Vsevolod I. Kiselev, Eugenii S. Severin, Victor A. Kabanov

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

A tool was developed for enhancement of plasmid penetration into an intact cell, based on increasing DNA hydrophobicity via inclusion into a soluble interpolyelectrolyte complex (IPC) with polycations. The characteristics of formation of DNA IPC with synthetic polycations [poly(N‐ethyl‐4‐vinylpyridinium)bromide (PVP) and PVP modified with 3% of N‐cetyl‐4‐vinylpyridinium units (PVP‐C)] were studied using ultracentrifugation and polyacrylamide gel electrophoresis methods. The conditions were established under which the mixing of DNA and polycation aqueous solutions results in the selfassembly of soluble IPC species. Incorporation of DNA into IPC results in the enhancement of DNA binding with isolated Bacillus subtilis membranes. A considerable increase in the efficiency of transformation of B. subtilis cells with pBC16 plasmid resulted from incorporation of the plasmid into the IPC with PVP and CVP.

Original languageEnglish (US)
Pages (from-to)1437-1443
Number of pages7
JournalBiopolymers
Volume31
Issue number12
DOIs
StatePublished - Oct 15 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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