DNA markers define plastid haplotypes in Arabidopsis thaliana

Arun K. Azhagiri, Pal Maliga

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

To identify genetic markers in the Arabidopsis thaliana plastid genome (ptDNA), we amplified and sequenced the rpl2-psbA and rbcL-accD regions in 26 ecotypes. The two regions contained eight polymorphic sites including five insertions and/or deletions (indels) involving changes in the length of A or T mononucleotide repeats and three base substitutions. The 27 alleles defined 15 plastid haplotypes, providing a practical set of ptDNA markers for the Columbia, Landsberg erecta and Wassilewskija ecotypes that are commonly used in genetic studies and also for the C24 and RLD ecotypes that are the most amenable for cell culture manipulations.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalCurrent Genetics
Volume51
Issue number4
DOIs
StatePublished - Apr 2007

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Arabidopsis thaliana ecotypes
  • Plastid DNA polymorphism
  • Plastid genetic marker
  • Plastid haplotype

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