DNA-mediated gene transfer into epidermal cells using electroporation

M. Reiss, M. M. Jastreboff, J. R. Bertino, R. Narayanan

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10-6 cells per μg of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.

Original languageEnglish (US)
Pages (from-to)244-249
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number1
DOIs
StatePublished - May 29 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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