A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10-6 cells per μg of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 29 1986|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology