TY - JOUR
T1 - Domains in the XPA protein important in its role as a processivity factor
AU - Bartels, Claudine L.
AU - Lambert, Muriel W.
N1 - Funding Information:
This work was supported by Grant R01 ES011298 to M.W.L. from the NIH.
PY - 2007/4/27
Y1 - 2007/4/27
N2 - XPA is a protein essential for nucleotide excision repair (NER) where it is thought to function in damage recognition/verification. We have proposed an additional role, that of a processivity factor, conferring a processive mechanism of action on XPF and XPG, the endonucleases, involved in NER. The present study was undertaken to examine the domain(s) in the XPA gene that are important for the ability of the XPA protein to function as a processivity factor. Using site-directed mutagenesis, mutations were created in several of the exons of XPA and mutant XPA proteins produced. The results showed that the DNA binding domain of XPA is critical for its ability to act as a processivity factor. Mutations in both the zinc finger motif and the large basic cleft in this domain eliminated the ability of XPA to confer a processive mechanism of action on the endonucleases involved in NER.
AB - XPA is a protein essential for nucleotide excision repair (NER) where it is thought to function in damage recognition/verification. We have proposed an additional role, that of a processivity factor, conferring a processive mechanism of action on XPF and XPG, the endonucleases, involved in NER. The present study was undertaken to examine the domain(s) in the XPA gene that are important for the ability of the XPA protein to function as a processivity factor. Using site-directed mutagenesis, mutations were created in several of the exons of XPA and mutant XPA proteins produced. The results showed that the DNA binding domain of XPA is critical for its ability to act as a processivity factor. Mutations in both the zinc finger motif and the large basic cleft in this domain eliminated the ability of XPA to confer a processive mechanism of action on the endonucleases involved in NER.
KW - DNA repair
KW - Processive mechanism of action
KW - XPA protein
KW - Xeroderma pigmentosum
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U2 - 10.1016/j.bbrc.2007.02.125
DO - 10.1016/j.bbrc.2007.02.125
M3 - Article
C2 - 17349973
AN - SCOPUS:33947146069
SN - 0006-291X
VL - 356
SP - 219
EP - 225
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -