Domestication in murtilla (Ugni molinae) reduced defensive flavonol levels but increased resistance against a native herbivorous insect

Manuel Chacón-Fuentes, Leonardo Parra, Cesar Rodriguez-Saona, Ivette Seguel, Ricardo Ceballos, Andres Quiroz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Plant domestication can have negative consequences for defensive traits against herbivores, potentially reducing the levels of chemical defenses in plants and consequently their resistance against herbivores.We characterized and quantified the defensive flavonols frommultiple cultivated ecotypes with wild ancestors of murtilla, Ugni molinae Turcz, an endemic plant from Chile, at different times of the year, and examined their effects on a native insect herbivore, Chilesia rudis Butler (Lepidoptera: Arctiidae). We hypothesized that domestication results in a decrease in flavonol levels in U. molinae plants, and that this negatively affected C. rudis performance and preference. Ethanolic extracts were made from leaves, stems, and fruit of murtilla plants for flavonol analysis. Flavonols identified were kaempferol, quercetin, rutin, and quercetin 3-D-α-glucoside, the last two being themost abundant.More interestingly, we showed differences in flavonol composition between wild and cultivated U. molinae that persisted for most of the year. Relative amounts of all four flavonols were higher in wild U. molinae leaves; however, no differences were found in the stem and fruit between wild and cultivated plants. In choice and no-choice assays, C. rudis larvae gained more mass on, and consumed more leaf material of, wild as compared with cultivated U. molinae plants.Moreover, when applied to leaves, larvae ate more leaf material with increasing concentrations of each flavonol compound. Our study demonstrates that domestication in U. molinae reduced the amount of flavonols in leaves as well as the performance and preference of C. rudis, indicating that these compounds stimulate feeding of C. rudis.

Original languageEnglish (US)
Pages (from-to)627-637
Number of pages11
JournalEnvironmental Entomology
Volume44
Issue number3
DOIs
StatePublished - Jun 1 2015

Fingerprint

domestication
phytophagous insects
flavonols
insect
herbivore
leaves
herbivores
fruit
stem
quercetin
larva
chemical defense
ecotype
Arctiinae
ancestry
stems
fruits
larvae
kaempferol
rutin

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Insect Science

Keywords

  • Chilesia rudis
  • HPLC
  • choice assay
  • domestication
  • nochoice assay

Cite this

Chacón-Fuentes, Manuel ; Parra, Leonardo ; Rodriguez-Saona, Cesar ; Seguel, Ivette ; Ceballos, Ricardo ; Quiroz, Andres. / Domestication in murtilla (Ugni molinae) reduced defensive flavonol levels but increased resistance against a native herbivorous insect. In: Environmental Entomology. 2015 ; Vol. 44, No. 3. pp. 627-637.
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Domestication in murtilla (Ugni molinae) reduced defensive flavonol levels but increased resistance against a native herbivorous insect. / Chacón-Fuentes, Manuel; Parra, Leonardo; Rodriguez-Saona, Cesar; Seguel, Ivette; Ceballos, Ricardo; Quiroz, Andres.

In: Environmental Entomology, Vol. 44, No. 3, 01.06.2015, p. 627-637.

Research output: Contribution to journalArticle

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